| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kouser, Madeline E (8)
|
MASJAC |
55.26 |
48.52 |
-6.74 |
| 2 |
Reber, Peyton E (8)
|
MALAC |
|
49.74 |
- |
| 3 |
Taylor, Reagan E (8)
|
MAACAC |
|
53.37 |
- |
| 4 |
Murray, Ellie R (8)
|
MAGAAC |
51.62 |
53.52 |
1.90 |
| 5 |
Suchecki, Madison R (7)
|
MAEAAC |
|
55.60 |
- |
| 6 |
Pfautz, Mia R (8)
|
MALAC |
|
55.93 |
- |
| 7 |
Fuertes, Allyssa D (8)
|
MALAC |
|
57.02 |
- |
| 8 |
Bieg, Theresa B (8)
|
MASJAC |
1:08.20 |
58.27 |
-9.93 |
| 9 |
Martin, Allison K (7)
|
MALAC |
|
1:05.15 |
- |
| 10 |
Betancourt, Eliana T (8)
|
MASJAC |
1:22.57 |
1:10.64 |
-11.93 |
|
Mahaniah, Leyli K (7)
|
MAFSA |
|
DQ |
- |
|
Wyche-Greenidge, Alonnie N (7)
|
MASJAC |
|
DQ |
- |
|
Gordon, Pennelope J (8)
|
MAFSA |
|
NS |
- |